Dipicolinic Acid Release by Germinating Clostridium difficile Spores Occurs through a Mechanosensing Mechanism
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منابع مشابه
Dipicolinic Acid Release by Germinating Clostridium difficile Spores Occurs through a Mechanosensing Mechanism
Classically, dormant endospores are defined by their resistance properties, particularly their resistance to heat. Much of the heat resistance is due to the large amount of dipicolinic acid (DPA) stored within the spore core. During spore germination, DPA is released and allows for rehydration of the otherwise-dehydrated core. In Bacillus subtilis, 7 proteins are encoded by the spoVA operon and...
متن کاملCharacterization of Clostridium difficile Spores Lacking Either SpoVAC or Dipicolinic Acid Synthetase
UNLABELLED The spore-forming obligate anaerobe Clostridium difficile is a leading cause of antibiotic-associated diarrhea around the world. In order for C. difficile to cause infection, its metabolically dormant spores must germinate in the gastrointestinal tract. During germination, spores degrade their protective cortex peptidoglycan layers, release dipicolinic acid (DPA), and hydrate their c...
متن کاملInduced release of dipicolinic acid from spores of Bacillus megaterium.
Conditions under which dipicolinic acid may be released from bacterial spores probably relate to the chemical state of the dipicolinic acid in the spore, to its physical location in the spore, and to its influence on the physiological properties of the spore. The latter aspect embraces the relation between release of dipicolinic acid and the viability of the spore, its heat resistance, and its ...
متن کاملRapid determination of dipicolinic acid in the spores of Clostridium species by gas-liquid chromatography.
A gas-liquid chromatographic procedure has been developed to quantitate dipicolinic acid in bacterial spores. The culture, washed from a plate, was hydrolyzed with acid containing the internal standard, pyridine-2,4-dicarboxylate, and then extracted into methyl isobutyl ketone. The internal standard and dipicolinic acid were then extracted into a small volume of trimethylphenylammonium hydroxid...
متن کاملAerial Dissemination of Clostridium difficile spores
BACKGROUND Clostridium difficile-associated diarrhoea (CDAD) is a frequently occurring healthcare-associated infection, which is responsible for significant morbidity and mortality amongst elderly patients in healthcare facilities. Environmental contamination is known to play an important contributory role in the spread of CDAD and it is suspected that contamination might be occurring as a resu...
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ژورنال
عنوان ژورنال: mSphere
سال: 2016
ISSN: 2379-5042
DOI: 10.1128/msphere.00306-16